plasma lipid profiles total cholesterol  (Randox)

Journal: Toxicological Sciences

Article Title: Dioxin-like PCB 126 Increases Systemic Inflammation and Accelerates Atherosclerosis in Lean LDL Receptor-Deficient Mice

doi: 10.1093/toxsci/kfx275

Figure Lengend Snippet: PCB 126 accelerates atherosclerosis at 12 weeks in mice fed a high cholesterol diet. Male Ldlr–/– mice were fed a low fat, 0.15% cholesterol diet and administered 1 µmol/kg PCB 126 at weeks 2 and 4. Aortic roots were frozen and serially sectioned from the emergence of the 3 valves. On average, the mean of 6 sections per mouse was utilized for quantitation. A, Shown are Oil Red O stained aortic root sections of mice exposed to vehicle control or PCB 126 with associated lesion area quantification (n = 10 control, n = 7 PCB; p < .01; Student’s t-test). B, Shown is CD68 staining by IHC which illustrates the early nature of the lesions with evident macrophage infiltration. The box and whisker plots show individual data points and also the median, mean, and error bars for each group.

Article Snippet: Lipid profiles Plasma total cholesterol (Infinity Cholesterol, ThermoScientific, USA), triglyceride (TRIGS, Randox, UK), phospholipid (Phospholipids C, Wako Pure Chemical Industries, Japan), and free fatty acid (Reagent A/B, Wako, Pure Chemical Industries, Japan) concentrations were determined via colorimetric kits, as per the manufacturer’s instructions.

Techniques: Quantitation Assay, Staining, Control, Whisker Assay

Journal: Toxicological Sciences

Article Title: Dioxin-like PCB 126 Increases Systemic Inflammation and Accelerates Atherosclerosis in Lean LDL Receptor-Deficient Mice

doi: 10.1093/toxsci/kfx275

Figure Lengend Snippet: Analysis of circulating cytokines showed that PCB 126 increases systemic inflammation at 10 weeks in mice fed a high-cholesterol diet. Male Ldlr–/– mice were fed a low fat, 0.15% cholesterol diet and exposed to 1 µmol/kg PCB 126 at weeks 2 and 4. PCB exposure increased plasma levels of G-CSF, IFNγ, IL-1β, IL-12 (p70), and IL-13 compared with vehicle safflower oil control (magpix technology; p < .05). IL-10, IFNγ-IP-10, and TNFα were not significantly increased in PCB administered mice (p = .10). The box and whisker plots show individual data points and also the median, mean, and error bars for each group. Statistical significance was determined by Student’s t-test or Mann-Whitney rank sum test depending on normality and variance distributions.

Article Snippet: Lipid profiles Plasma total cholesterol (Infinity Cholesterol, ThermoScientific, USA), triglyceride (TRIGS, Randox, UK), phospholipid (Phospholipids C, Wako Pure Chemical Industries, Japan), and free fatty acid (Reagent A/B, Wako, Pure Chemical Industries, Japan) concentrations were determined via colorimetric kits, as per the manufacturer’s instructions.

Techniques: Clinical Proteomics, Control, Whisker Assay, MANN-WHITNEY

Journal: Toxicological Sciences

Article Title: Dioxin-like PCB 126 Increases Systemic Inflammation and Accelerates Atherosclerosis in Lean LDL Receptor-Deficient Mice

doi: 10.1093/toxsci/kfx275

Figure Lengend Snippet: Analysis of circulating hematological parameters showed that PCB 126 may lead to anemia of inflammation at 10 weeks in mice fed a high cholesterol diet. Male Ldlr–/– mice were fed a low fat, 0.15% cholesterol diet and exposed to 1 µmol/kg PCB 126 at weeks 2 and 4. Whole blood was collected at sacrifice and analyzed for blood cell parameters via hemavet instrumentation. PCB 126 exposure increased circulating platelet counts and decreased circulating red blood cell counts in conjunction with a decreased hematocrit and hemoglobin concentration (n = 10 per group; p < .05). The box and whisker plots show individual data points and also the median, mean, and error bars for each group. Statistical significance was determined by Student’s t-test or Mann-Whitney rank sum test depending on normality and variance distributions.

Article Snippet: Lipid profiles Plasma total cholesterol (Infinity Cholesterol, ThermoScientific, USA), triglyceride (TRIGS, Randox, UK), phospholipid (Phospholipids C, Wako Pure Chemical Industries, Japan), and free fatty acid (Reagent A/B, Wako, Pure Chemical Industries, Japan) concentrations were determined via colorimetric kits, as per the manufacturer’s instructions.

Techniques: Concentration Assay, Whisker Assay, MANN-WHITNEY

Journal: Toxicological Sciences

Article Title: Dioxin-like PCB 126 Increases Systemic Inflammation and Accelerates Atherosclerosis in Lean LDL Receptor-Deficient Mice

doi: 10.1093/toxsci/kfx275

Figure Lengend Snippet: Dioxin-like PCB 126 is associated with increased PB inflammatory cells. Exposure to PCB 126 increases circulating levels of monocytes, macrophages, and neutrophils at 14 weeks in mice fed a high-cholesterol diet. PB was collected at sacrifice and blood cell types were isolated by flow cytometry. Quantitative analyses of monocyte subpopulations in PB demonstrating an increase in PB monocyte subpopulations (Panel A). Similarly, significant increase in neutrophils and inflammatory macrophages was observed in PB samples (Panel B). Representative FACS plots demonstrating the gating strategy for Ly6Chi (CD45+/CD115+/Ly6C-G+), Ly6Clo (CD45+/CD115+/Ly6C-G−) monocytes and neutrophils (CD45+/CD115−/Ly-6 G+/C+) (Panel C). Data are presented as mean ± SEM (n = 5 per group). Statistical significance was determined by Student’s t-test or Mann-Whitney rank sum test depending on normality and variance distributions.

Article Snippet: Lipid profiles Plasma total cholesterol (Infinity Cholesterol, ThermoScientific, USA), triglyceride (TRIGS, Randox, UK), phospholipid (Phospholipids C, Wako Pure Chemical Industries, Japan), and free fatty acid (Reagent A/B, Wako, Pure Chemical Industries, Japan) concentrations were determined via colorimetric kits, as per the manufacturer’s instructions.

Techniques: Isolation, Flow Cytometry, MANN-WHITNEY

Journal: Toxicological Sciences

Article Title: Dioxin-like PCB 126 Increases Systemic Inflammation and Accelerates Atherosclerosis in Lean LDL Receptor-Deficient Mice

doi: 10.1093/toxsci/kfx275

Figure Lengend Snippet: Exposure to PCB 126 modulates hepatic lipid accumulation at 14 weeks in mice fed a high cholesterol diet. Male Ldlr–/– mice were fed a low fat, 0.15% cholesterol diet and exposed to 1 µmol/kg PCB 126 at weeks 2 and 4. All mRNA values were determined using the relative quantification method (ΔΔCt) and normalized to control. Hprt1 was used as the housekeeping gene for all mRNA quantifications. A, PCB 126 increased hepatic mRNA levels of fatty acid translocase (CD36), INSIG-1, IL-1β, LPL, Phospholipase A2 Group IVA, and downregulated Fads1, Scd1, Gyk, Solute Carrier Family 16 Member 6, and leukotriene A4 hydrolase. Data are presented as mean ± SEM (n = 5 per group; Student’s t-test). B, Shown are representative H&E stained hepatic sections which illustrate the observed PCB-induced significant microvesicular fatty change in centrilobular regions.

Article Snippet: Lipid profiles Plasma total cholesterol (Infinity Cholesterol, ThermoScientific, USA), triglyceride (TRIGS, Randox, UK), phospholipid (Phospholipids C, Wako Pure Chemical Industries, Japan), and free fatty acid (Reagent A/B, Wako, Pure Chemical Industries, Japan) concentrations were determined via colorimetric kits, as per the manufacturer’s instructions.

Techniques: Quantitative Proteomics, Control, Staining